In court papers, Madonna said the auction of her belongings is unauthorized and an invasion of her privacy.
"The fact that I have attained celebrity status as a result of success in my career does not obviate my right to maintain my privacy, including with regard to highly personal items," the AP reported.
"I understand that my DNA could be extracted from a piece of my hair. It is outrageous and grossly offensive that my DNA could be auctioned for sale to the general public."
She's referring to the hairbrush, one of the items that was up for bid from Gotta Have It! Collectibles, the company holding the auction.
The lawsuit named Darlene Lutz, according to entertainment website Page Six.
In the suit, the pop star called Lutz “a former friend” and “frequent overnight guest at Madonna’s Miami home when she was “not in residence.”
The accusations will be "vigorously challenged and refuted" in court, a spokesman for Lutz said.
A hearing on the case is scheduled fro September.