HIT WITH SANCTIONS

Here are the Russian officials targeted:

Oleg Belavantsev: Russian President Vladimir Putin's envoy to Crimea

Sergei Chemezov: Head of Rostec, an immense state-owned holding company formed around arms exporter Rosoboronexpor

Dmitry Kozak: A deputy prime minister since 2008

Yevgeny Murov: Head of the Federal Protective Service since 2000

Alexei Pushkov: Chairman of the international affairs committee in the lower house of Russia's parliament

Igor Sechin: President of state-owned oil company Rosneft

Vyacheslav Volodin: First deputy chief of the Kremlin staff since late 2011

— Associated Press

The mayor of Ukraine’s second-largest city was shot in the back Monday, and hundreds of men attacked a peaceful pro-Ukraine rally with batons, bricks and stun grenades, wounding dozens as tensions soared in Ukraine’s volatile east.

One presidential candidate said the mayor was deliberately targeted in an effort to destabilize the entire city of Kharkiv, a hub of 1.5 million people.

Russia’s defense chief, meanwhile, assured U.S. Defense Secretary Chuck Hagel in a telephone call that Russia would not invade Ukraine, the Pentagon said.

Armed insurgents tacitly backed by Moscow are seeking more autonomy in eastern Ukraine — and possibly even independence or annexation with Russia. Ukraine’s acting government and the West have accused Russia of orchestrating the unrest, which they fear Moscow could use as a pretext for an invasion.

Ratcheting up the pressure, President Barack Obama’s government levied new sanctions on seven Russian officials and 17 companies with links to President Vladimir Putin’s inner circle. The U.S. also revoked licenses for some high-tech items that could be used by the Russian military.

In Brussels, the European Union moved to add 15 more officials to its Russian sanctions list to protest Moscow’s meddling in Ukraine. That decision, reached by the ambassadors to the EU’s 28 nations, was being formally confirmed by the EU’s governments, officials said.

In the eastern city of Donetsk, about 1,000 demonstrators carrying Ukrainian flags marched through the streets to hold a pro-Ukrainian rally Monday night. They were attacked by several hundred armed men shouting “Russia!”

Police attempted to hold the pro-Russia men back, but then largely stood aside as dozens of protesters were battered.

Hennady Kernes, the mayor of Kharkiv, was shot in the back Monday morning while cycling on the outskirts of the city, his office said. He underwent surgery and was reported by the hospital to be in “grave but stable” condition.

Officials have not commented on who could be behind the attack on the mayor — but Kernes was a man who could have angered both sides.

Kernes’ friend and former Kharkiv governor, Mykhailo Dobkin, told journalists the attackers had aimed at Kernes’ heart and wanted to kill him to destabilize the city.

“If you want to know my opinion, they were shooting not at Kernes, but at Kharkiv,” he said.

Dobkin is among several candidates running in Ukraine’s May 25 presidential election, which the interim government says Russia is trying to derail.

Kharkiv is in eastern Ukraine, where pro-Russia gunmen have seized government buildings and police stations and set up roadblocks to demand greater autonomy or even annexation by Russia.

Elsewhere in the east, pro-Russia militants wearing masks gained another foothold, seizing a city hall building and police station in the city of Kostyantynivka, 100 miles from the Russian border. The city is 22 miles south of Slovyansk, a major city that has been in the hands of insurgents for more than three weeks.

Moscow has repeatedly pushed for a referendum on federal autonomy in Ukraine, but Kiev and its Western allies have refused, accusing Russia of fomenting separatist sentiment to foil the May presidential vote.

However, Justice Minister Petro Petrenko said the parliament in Kiev will hold a debate today on the idea of a referendum, Interfax news agency reported.